International Journal of Hematology and Oncology 2024, Vol 34, Num 1 Page(s): 116-122
The Effect of Calcium Canal Blocker, Verapamil, on Cell Cycle and Apoptosis in Acute Myeloid Leukemia Cancer Cell Line (HL-60)

Gülper NACARKAHYA1, Mehmet OZASLAN2, Mehmet YILMAZ3, I. Didem KARAGOZ2, Ibrahim H. KILIC2, Ebru TEMIZ1, Beyhan CENGIZ4

1Gaziantep University, Faculty of Medicine, Department of Medical Biology, Gaziantep, TURKEY
2Gaziantep University, Faculty of Science, Department of Molecular Biology, Gaziantep, TURKEY
3Gaziantep University, Faculty of Medicine, Department of Hematology, Gaziantep, TURKEY
4Gazi University, Faculty of Medicine, Department of Medical Genetics, Ankara, TURKEY

Keywords: AML, Verapamil, Cell cycle, Apoptosis
Acute Myeloid Leukemia (AML) is a hematopoietic stem cell disorder. Many of the tumor development steps are processes regulated by Ca2+ dependent signaling pathways found in tumor growth. Calcium channels, which can be blocked by pharmacological agents such as verapamil, are important in the regulation of intracellular Ca2+ homeostasis. The failure to stop cell cycle and to achieve programmed cell death are the most important factors in AML or other types of cancers and are considered to be the real cause of cancer. Our aim in this study was to investigate the effect of verapamil on apoptosis and cell cycle in AML cell line, HL-60, using flow cytometry. The effective dose of verapamil was determined as 50 mg / ml by MTT method, and was applied to HL-60 cells, subsequently, they were assessed for apoptosis and cell cycle. After 50 mg / ml of verapamil application, apoptotic cell ratio was found to be 77.7% after 24 hours (p< 0.05). In cell cycle analysis, the ratio of diploid cells decreased in G1 phase and increased in S phase, and the ratio of aneuploid cells decreased in G1 phase (p< 0.05) and increased in S phase (p> 0.05) in verapamil-untreated group when compared to verapamil-treated group.