International Journal of Hematology and Oncology 2023, Vol 33, Num 4 Page(s): 009-014
Effects of Ankaferd Blood Stopper on Nerve Conductance: An experimental study on the Rat Sciatic Nerve

Alper AKTAS1, Nuray ER1, Mehmet A. ONUR2, Gamze TAN2, Mutlu HAYRAN3

1Hacettepe University Faculty of Dentistry Department of Oral Maxillofacial Surgery, Ankara, TURKEY
2Hacettepe University Faculty of Science Department of Biology, Ankara, TURKEY
3Hacettepe University Faculty of Medicine Department of Preventive Oncology Institute of Oncology, Ankara, TURKEY

Keywords: Haemostatic, Neural conduction, Nervus ischiadicus, Action potentials
The aim of this study was to evaluate the effect of AnkaferdBloodStopper® (ABS) on nerve conductance. ABS consists of traditional haemostatic ingredients, and has been officially approved for topical use in the management of external hemorrhage and dental surgical procedures. The basic mechanism of action for ABS is the formation of an encapsulated protein network that provides focal points for vital erythrocyte aggregation.

Total of 6 albino rats were used for nerve conduction experiments. Isolated rat sciatic nerves were placed in two suction electrodes in a pyrex bath containing a tyrode solution. Concentration-dependent evoked compound action potentials (cAP) of the nerves were recorded for 60 minutes within the ABS solution. The readings were conducted for 60 more minutes after washing the nerves. A significant reduction cAP voltage in 1000 μl test groups was recorded.

The pairwise comparisons showed that the only significant differences were between 1000uL and the rest of the solutions, but this effect was totally reversible after washing with tyrode solution at 120 min readings. No significant difference exists among groups for any other parameters. Observation of recovery of the cAPs on washed specimens, demonstrating that ABS has a reversible adverse effect on nerve conductance irrespective of the concentration applied.