International Journal of Hematology and Oncology
2023, Vol 33, Num 4 Page(s): 057-060
COMPARISON OF MICROSCOPIC, FLOW CYTOMETRIC AND COULTER STKS ANALYSER METHODS FOR RETICULOCYTE COUNTING
A. VAHAP ASLAN1, GÜLCİHAN DEMİREL1, ZAFER GÜLBAŞ1
Osmangazi Üniversitesi Tıp Fakültesi İç Hastalıklan Anabilim dalı, Hematoloji Bilim Dalı, ESKİŞEHİR
Keywords: reticulocyte, microscopic, flow cytometry, coulter stks analyser
We compared microscopic (brilliant cresyl blue stain), flow cytometric (thiazole orange technic), coulter STKS (VCS technology) reticulocyte count methods. There were good correlations between microscopic and flow cytometric reticulocyte counts (r=0.94), microscopic and coulter STKS analyser reticulocyte counts (r=0.84), flow cytometric and coulter STKS analyser reticulocyte counts (r=0.83). Coefficient of variation (CV) were 14.6% for microscopic method. 9.7% for flow cytometric method, 11.8% for coulter STKS method. Our data suggests that flow cytometric and coulter STKS analyser reticulocyte count methods are reliabte for routine reticulocyte count.
A. VAHAP ASLAN1, GÜLCİHAN DEMİREL1, ZAFER GÜLBAŞ1
Osmangazi Üniversitesi Tıp Fakültesi İç Hastalıklan Anabilim dalı, Hematoloji Bilim Dalı, ESKİŞEHİR
Keywords: reticulocyte, microscopic, flow cytometry, coulter stks analyser
We compared microscopic (brilliant cresyl blue stain), flow cytometric (thiazole orange technic), coulter STKS (VCS technology) reticulocyte count methods. There were good correlations between microscopic and flow cytometric reticulocyte counts (r=0.94), microscopic and coulter STKS analyser reticulocyte counts (r=0.84), flow cytometric and coulter STKS analyser reticulocyte counts (r=0.83). Coefficient of variation (CV) were 14.6% for microscopic method. 9.7% for flow cytometric method, 11.8% for coulter STKS method. Our data suggests that flow cytometric and coulter STKS analyser reticulocyte count methods are reliabte for routine reticulocyte count.