International Journal of Hematology and Oncology 2019, Vol 29, Num 3 Page(s): 037-046
In Vitro Cytotoxic and Proapoptotic Activities of Anatolian Macrovipera Lebetina Obtusa (Dwigubski, 1832) Crude Venom on Cultured K562 Human Chronic Myelogenous Leukemia Cells

Faruk SUZERGOZ1, Nasit IGCI2, Cemal CAVUS1, Mehmet Z. YILDIZ3, Mehmet B. COSKUN1, Bayram GOCMEN4

1Harran University Faculty of Arts and Science, Department of Biology, Sanliurfa, TURKEY
2Nevşehir Hacı Bektas Veli University Faculty of Arts and Sciences, Department of Molecular Biology and Genetics, Nevsehir, TURKEY
3Adıyaman University Faculty of Arts and Science, Department of Biology, Adıyaman, TURKEY
4Ege University Faculty of Science, Department of Biology, Izmir, TURKEY

Keywords: Anticancer, Apoptosis, Blunt-nosed viper, Macrovipera lebetina obtusa, Snake venom
In the context of searching for anticancer compounds in natural products, snake venom is one of the important sources for peptide/ protein based bioactive molecules. Proteins and peptides with anticancer activity were purified and identified from snake venoms. The aim of the present study was to determine the in vitro cytotoxicity of Macrovipera lebetina obtusa (Blunt-Nosed Viper) crude venom from southeastern Anatolia against K562 human chronic myelogenous leukemia (CML) cells by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Adenosine tripohsphate (ATP) assays. Additionally, the apoptosis induction was assessed by morphological evaluation and immunohistochemical analysis for activated caspase-3. For histopahtological evaluation, haematoxylineosin, giemsa and papanicolau stains were used in combination. M. l. obtusa venom showed dose-dependent toxicity against K562 cells after 72 h treatment with different concentrations of crude venom. IC50 values were 0.45 and 0.37 μg/mL for MTT and ATP assays, respectively. Nuclear fragmentation and condensation, apoptotic bodies and activation of caspase-3, as an induction of apoptosis were also observed in K562 cells. Since apoptosis-inducing compounds are important for the treatment of cancer, further studies on Anatolian M. l. obtusa venom could result in the purification and identification of new proteins and peptides, which might have therapeutic value for the treatment of CML.