International Journal of Hematology and Oncology
2023, Vol 33, Num 4 Page(s): 158-165
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Changes of the Expressions of Orphan and gon-ADAMTS in Chondrosarcoma Cells
Bunyamin ISIK1, Haci K. ERDEMLI2, Ismail COMERTOGLU3, Sumeyya AKYOL4, Ridvan FIRAT5, Mehmet KAYA6, Omer AKYOL7, Kadir DEMIRCAN4
1Hacettepe University Medical Faculty of Medicine, Departments of Family Medicine, Ankara
2Corum Training and Research Hospital, Department of Biochemistry Laboratory, Corum
3Mevlana University, Faculty of Medicine, Department of Medical Genetics, Konya
4Turgut Ozal University, Faculty of Sciences Departments of Medical Biology
5Golbasi State Hospital, Division of Medical Biochemistry, Ankara
6Turgut Ozal University, Faculty of Medicine, Department of Public Health, Ankara
7Hacettepe University Faculty of Medicine, Department of Medical Biochemistry, Ankara, TURKEY
Keywords: ADAMTS; Chondrosarcoma; Insulin; qRT-PCR, OUMS-27
Some of A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) enzymes have been suggested to facilitate invasion and metastasis in cancer. ADAMTS20 is called gon-ADAMTS and ADAMTS10 and -17 are called orphan ADAMTSs. ADAMTS20 degrades versican and aggrecan in extracellular matrix. We aimed to investigate the effects of insulin on ADAMTS10,-17 and -20 in OUMS-27 chondrosarcoma cells. OUMS-27 cells were cultured in Dulbecco’s modified Eagle’ medium (DMEM) containing 10 μg/ mL insulin. The medium was changed every other day up to 11th day. Cells were harvested at 1, 3, 7, and 11th days and RNA isolation was performed at appropriate times according to study setup. The levels of RNA expression of ADAMTS10,-17 and -20 were estimated by qRT-PCR using appropriate primers. ADAMTS10 mRNA expression gradually decreased within 7 days after insulin induction compared to control group. There was a significant difference between control and Day 7 groups (p=0.021) as well as Day 1 and Day 7 groups (p=0.028). ADAMTS17 mRNA expression increased right after insulin induction at day 1 compared to control group and protected its high levels throughout insulin application. The most evident and statistically significant increase in mRNA concentration was observed at day 7 after insulin induction (p= 0.014). Our results demonstrated that ADAMTS10,-17 and -20 might have a role in cancer progression. Although functions of ADAMTS10 and -17 are not known, their expression levels have changed in chondrosarcoma cell line. Further studies are needed to characterize chondrosarcoma cells because of the possible association of cancer progression and ADAMTS proteins.
Bunyamin ISIK1, Haci K. ERDEMLI2, Ismail COMERTOGLU3, Sumeyya AKYOL4, Ridvan FIRAT5, Mehmet KAYA6, Omer AKYOL7, Kadir DEMIRCAN4
1Hacettepe University Medical Faculty of Medicine, Departments of Family Medicine, Ankara
2Corum Training and Research Hospital, Department of Biochemistry Laboratory, Corum
3Mevlana University, Faculty of Medicine, Department of Medical Genetics, Konya
4Turgut Ozal University, Faculty of Sciences Departments of Medical Biology
5Golbasi State Hospital, Division of Medical Biochemistry, Ankara
6Turgut Ozal University, Faculty of Medicine, Department of Public Health, Ankara
7Hacettepe University Faculty of Medicine, Department of Medical Biochemistry, Ankara, TURKEY
Keywords: ADAMTS; Chondrosarcoma; Insulin; qRT-PCR, OUMS-27
Some of A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) enzymes have been suggested to facilitate invasion and metastasis in cancer. ADAMTS20 is called gon-ADAMTS and ADAMTS10 and -17 are called orphan ADAMTSs. ADAMTS20 degrades versican and aggrecan in extracellular matrix. We aimed to investigate the effects of insulin on ADAMTS10,-17 and -20 in OUMS-27 chondrosarcoma cells. OUMS-27 cells were cultured in Dulbecco’s modified Eagle’ medium (DMEM) containing 10 μg/ mL insulin. The medium was changed every other day up to 11th day. Cells were harvested at 1, 3, 7, and 11th days and RNA isolation was performed at appropriate times according to study setup. The levels of RNA expression of ADAMTS10,-17 and -20 were estimated by qRT-PCR using appropriate primers. ADAMTS10 mRNA expression gradually decreased within 7 days after insulin induction compared to control group. There was a significant difference between control and Day 7 groups (p=0.021) as well as Day 1 and Day 7 groups (p=0.028). ADAMTS17 mRNA expression increased right after insulin induction at day 1 compared to control group and protected its high levels throughout insulin application. The most evident and statistically significant increase in mRNA concentration was observed at day 7 after insulin induction (p= 0.014). Our results demonstrated that ADAMTS10,-17 and -20 might have a role in cancer progression. Although functions of ADAMTS10 and -17 are not known, their expression levels have changed in chondrosarcoma cell line. Further studies are needed to characterize chondrosarcoma cells because of the possible association of cancer progression and ADAMTS proteins.
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