International Journal of Hematology and Oncology 2018, Vol 28, Num 1 Page(s): 204-208
The Effect of Clarithromycin+amoxicillin Treatment on Oxidative DNA Damage in Helicobacter Pylori Infected Cases

Yıldız DİNÇER1, Tulay AKÇAY1, E. İlker SAYGILI1, E. Yeliz ERSOY2, Osman TORTUM2

1İstanbul Üniversitesi Cerrahpaşa Tıp Fakültesi Biyokimya Anabilimdalı, İSTANBUL
2İstanbul Üniversitesi Cerrahpaşa Tıp Fakültesi Genel Cerrahi Anabilimdalı, İSTANBUL

Keywords: Helicobacter pylori, oxidative stress, comet assay, DNA strand breaks, fpg-sensitive sites
Epidemiological studies revealed increased gastric cancer risk in helicobacter pylori-infected cases and Helicobacter pylori-mediated oxidative DNA damage is suggested as responsible factor. In order to examine the effect of clarithromycin+ amoxicillin treatment on oxidative DNA damage in helicobacter pylori-infected cases, DNA damage in the basis of frequency of DNA strand breaks and formamidopyrimidine DNA glycosylase (fpg)-sensitive sites were determined before and after two weeks of treatment with clarithromycin (1 g/day) and amoxicillin (1 g/day). The frequency of fpg-sensitive sites determined by the comet assay was found to be decreased after the treatment (p<0.001) but frequency of DNA strand breaks was unchanged. Frequency of fpg-sensitive sites was found to be positively correlated with age (r: 0.59; p<0.05). Our data suggested that treatment of helicobacter pylori by clarithromycin +amoxicillin may prevent the helicobacter pylori-mediated gastric cancer risk by decreasing oxidative DNA damage in helicobacter pylori infected patients.