International Journal of Hematology and Oncology 2021, Vol 31, Num 4 Page(s): 178-184
THE ANTITUMORAL AND BIOCHEMICAL EFFECTS OF GOSSYPOL ON HUMAN CERVIX CANCER CELL LINE ME-180

Esra A. AYDEMİR1, Kayahan FIŞKIN1

Akdeniz University, Faculty of Arts and Sciences, Department of Biology, ANTALYA

Keywords: Gossypol, Apoptosis, Catalase, Glutathione reductase, Glutathione-s-transferase
Gossypol, a polyphenolic compound extracted from cotton plants, has been found to be an anticancerogenic agent. The aim of our study was to investigate whether gossypol induced cell death on ME-180 cervix cancer cells, and whether it was a potent inhibitor of some antioxidant enzymes, like catalase, glutathione reductase and glutathione-S-transferase.

The cells were incubated with four different doses (5,10,15 and 20 µM ) for 24, 48 and 72 hours. After gossypol treatment, the cytotoxic effects were measured with MTT tests. Using DNA agarose gel electrophoresis, cellular internucleosomal DNA fragmentation of the cells treated with gossypol and untreated were examined. Consequently, gossypol caused different fragmentation on tumour cells due to apoptosis. Gossypol was found to be a potent inhibitor of catalase, glutathione reductase and glutathione-S-transferase.