International Journal of Hematology and Oncology
2023, Vol 33, Num 4 Page(s): 172-177
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ALTERATION OF TUMOR GLUCOSE METABOLISM AFTER RADIOTHERAPY IN MCF-7 BREAST CANCER CELL LINES
Mustafa CENGİZ1, Hakan BOYUNAĞA2, Ferah YILDIZ1, A. Uğur URAL3, İ. Lale ATAHAN1
1Hacettepe Üniversitesi Tıp Fakültesi Radyasyon Onkolojisi Anabilim Dalı, ANKARA
2Kırıkkale Üniversitesi Tıp Fakültesi Biyokimya Anabilim Dalı, KIRIKKALE
3Gülhane Askeri Tıp Akademisi, Hematoloji Bilim Dalı, ANKARA
Keywords: Radiation, Tumor metabolism, Hypoxia, MCF-7 cell line
Cancer cells utilize anaerobic glycolytic way to compensate their faster metabolism when compared to normal cells. The purpose of this study is to investigate the effect of radiation on tumor metabolism.
MCF-7 breast cancer cell lines were divided into 4 groups, including 2 control groups and aerobic and anaerobic study groups (were irradiated 600 cGy by Co-60 teletherapy unit), incubated with radiolabelled glucose for 4 hours. One control group was for aerobic, and the other was for anaerobic group after KCN addition. Radiolabelled CO2 produced by the cells was isolated and collected in specially designed sintillation vials. In supernatant the measurements of other end-products of carbohydrate catabolism including lactate, pyruvate, acetate were performed on a liquid scintillation analyzer after they were collected via anion-exchange chromatography. Finally glucose in supernatant was measured enzymatically by glucose oxidase method. Glycogen consumption and lactate production were significantly higher in anaerobic and radiation groups (p<0.01). Whereas CO2 production was significantly higher in control group (p<0.01). Taken all results together radiation lead tumor cells more anaerobic glycolysis with high glycogen consumption, high lactate production and low CO2 production. Radiation itself has led tumor cells to produce energy by anaerobic glycolysis, meaning radiation exposed cells become more hypoxic.
Mustafa CENGİZ1, Hakan BOYUNAĞA2, Ferah YILDIZ1, A. Uğur URAL3, İ. Lale ATAHAN1
1Hacettepe Üniversitesi Tıp Fakültesi Radyasyon Onkolojisi Anabilim Dalı, ANKARA
2Kırıkkale Üniversitesi Tıp Fakültesi Biyokimya Anabilim Dalı, KIRIKKALE
3Gülhane Askeri Tıp Akademisi, Hematoloji Bilim Dalı, ANKARA
Keywords: Radiation, Tumor metabolism, Hypoxia, MCF-7 cell line
Cancer cells utilize anaerobic glycolytic way to compensate their faster metabolism when compared to normal cells. The purpose of this study is to investigate the effect of radiation on tumor metabolism.
MCF-7 breast cancer cell lines were divided into 4 groups, including 2 control groups and aerobic and anaerobic study groups (were irradiated 600 cGy by Co-60 teletherapy unit), incubated with radiolabelled glucose for 4 hours. One control group was for aerobic, and the other was for anaerobic group after KCN addition. Radiolabelled CO2 produced by the cells was isolated and collected in specially designed sintillation vials. In supernatant the measurements of other end-products of carbohydrate catabolism including lactate, pyruvate, acetate were performed on a liquid scintillation analyzer after they were collected via anion-exchange chromatography. Finally glucose in supernatant was measured enzymatically by glucose oxidase method. Glycogen consumption and lactate production were significantly higher in anaerobic and radiation groups (p<0.01). Whereas CO2 production was significantly higher in control group (p<0.01). Taken all results together radiation lead tumor cells more anaerobic glycolysis with high glycogen consumption, high lactate production and low CO2 production. Radiation itself has led tumor cells to produce energy by anaerobic glycolysis, meaning radiation exposed cells become more hypoxic.
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