International Journal of Hematology and Oncology
2023, Vol 33, Num 4 Page(s): 119-125
INTERACTION OF CHRONIC B-CELL LEUCEMIA CELLS WITH CD4+ AND CD8+ T CELLS IN VITRO AND THE EFFECT OF INTERFERON-ALPHA
BÜLENT ÜNDAR1, HAYRİ G. ÖZSAN1, MEHMET A. ÖZCAN1, HALİL ATES1, FATİH DEMİRKAN1, FİLİZ VURAL1, İLHAN ÖZTOP1
Dokuz Eylül Üniversitesi Tıp Fakültesi, İç Hastalıkları Ana Bilim Dalı, Hematoloji-Onkoloji Bilim Dalı, İZMİR
Keywords: cll, ifn-alpha, t-cells
Altough interferon-Alpha (IFN-Alpha) is an effective agent in chronic lymphocytic leukemia (CLL) in vivo, it protects CLL cells from apoptosis in vitro. In our study we investigated the in vitro effectiveness of IFN-Alpha in B-CLL. Positive cell selection for CD4+ and CD8+ T cells was performed from peripheral blood mononuclear cells of 2 CLL patients and 2 healthy controls. B-CLL cells were cultured in mediums containing or not containing IFN-Alpha in the following conditions: 1) B-CLL cells only 2) with autologous or allogeneic CD4+ and CD8+ cells in transwell system 3) with autologous CD4+ and CD8+ cells in a cell to cell direct contact system. Fas (CD95), CD40 and annexin-V expressions of the cells were studied by flow-cytometry at the beginning and after 72 hours of culture. Both baseline and post-culture fas expressions were low in B-CLL cells. Post culture CD40 expressions of B-CLL cells decreased significantly in all culture conditions. When B-CLL cells were in direct contact with T cells, annexin V expressions were decreased. However addition of IFN-Alpha to these mediums increased annexin-V expression B-CLL cells. This increment was in parellel to the increased expression of CD95 and annexin V in CD8+ T cells. The effectiveness of IFN-Alpha in CLL may be due to its capability of increasing apoptosis in T cells.
BÜLENT ÜNDAR1, HAYRİ G. ÖZSAN1, MEHMET A. ÖZCAN1, HALİL ATES1, FATİH DEMİRKAN1, FİLİZ VURAL1, İLHAN ÖZTOP1
Dokuz Eylül Üniversitesi Tıp Fakültesi, İç Hastalıkları Ana Bilim Dalı, Hematoloji-Onkoloji Bilim Dalı, İZMİR
Keywords: cll, ifn-alpha, t-cells
Altough interferon-Alpha (IFN-Alpha) is an effective agent in chronic lymphocytic leukemia (CLL) in vivo, it protects CLL cells from apoptosis in vitro. In our study we investigated the in vitro effectiveness of IFN-Alpha in B-CLL. Positive cell selection for CD4+ and CD8+ T cells was performed from peripheral blood mononuclear cells of 2 CLL patients and 2 healthy controls. B-CLL cells were cultured in mediums containing or not containing IFN-Alpha in the following conditions: 1) B-CLL cells only 2) with autologous or allogeneic CD4+ and CD8+ cells in transwell system 3) with autologous CD4+ and CD8+ cells in a cell to cell direct contact system. Fas (CD95), CD40 and annexin-V expressions of the cells were studied by flow-cytometry at the beginning and after 72 hours of culture. Both baseline and post-culture fas expressions were low in B-CLL cells. Post culture CD40 expressions of B-CLL cells decreased significantly in all culture conditions. When B-CLL cells were in direct contact with T cells, annexin V expressions were decreased. However addition of IFN-Alpha to these mediums increased annexin-V expression B-CLL cells. This increment was in parellel to the increased expression of CD95 and annexin V in CD8+ T cells. The effectiveness of IFN-Alpha in CLL may be due to its capability of increasing apoptosis in T cells.