International Journal of Hematology and Oncology
2025, Vol 35, Num 1 Page(s): 264-268
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Renin-Angiotensin System (RAS) Expressions in Myeloid Leukemic Cell Lines
Burak UZ1, Suzin C. TATONYAN2, Muge SAYITOGLU2, Yucel ERBILGIN2, Ozden HATIRNAZ NG2, Yahya BUYUKASIK1, Salih AKSU1, Nilgun SAYINALP1, Hakan GOKER1, Osman I. OZCEBE1, Ugur OZBEK2, Ibrahim C. HAZNEDAROGLU1
1Hacettepe University Department of Internal Medicine, Hematology Unit, Ankara, TURKEY
2Istanbul University Institute for Experimental Medicine Research, Genetics Department, Istanbul, TURKEY
Keywords: Renin-angiotensin system, KG-1 AML cell line, HL-60 promyelocytic cell line, mRNA expressions
The aim of this study is to search critical renin-angiotensin system (RAS) elements in myeloid leukemic cell lines. Human acute myeloid leukemia (AML) cell lines, KG-1 and HL-60, were cultured. We searched for the gene expression of the major RAS components in KG-1 and HL-60 cell lines by quantitative real-time polymerase chain reaction analysis (qRT-PCR). Angiotensin-converting enzyme I (ACE I) and ACE II mRNA expressions were detected in KG-1 AML cell line. Relative expression of ACE I was higher than ACE II expression in this myeloid leukemic cells. Likewise, RENIN, angiotensinogen (ANGTS), and ACE I mRNA expressions were detected in HL-60 promyelocytic cell line. Relative RENIN expression was the highest, whereas ACE I expression was the lowest in HL- 60 neoplastic myeloid cells. These findings indicate that there is a biologically active local RAS in the hematopoietic system in normal and pathological states.
Burak UZ1, Suzin C. TATONYAN2, Muge SAYITOGLU2, Yucel ERBILGIN2, Ozden HATIRNAZ NG2, Yahya BUYUKASIK1, Salih AKSU1, Nilgun SAYINALP1, Hakan GOKER1, Osman I. OZCEBE1, Ugur OZBEK2, Ibrahim C. HAZNEDAROGLU1
1Hacettepe University Department of Internal Medicine, Hematology Unit, Ankara, TURKEY
2Istanbul University Institute for Experimental Medicine Research, Genetics Department, Istanbul, TURKEY
Keywords: Renin-angiotensin system, KG-1 AML cell line, HL-60 promyelocytic cell line, mRNA expressions
The aim of this study is to search critical renin-angiotensin system (RAS) elements in myeloid leukemic cell lines. Human acute myeloid leukemia (AML) cell lines, KG-1 and HL-60, were cultured. We searched for the gene expression of the major RAS components in KG-1 and HL-60 cell lines by quantitative real-time polymerase chain reaction analysis (qRT-PCR). Angiotensin-converting enzyme I (ACE I) and ACE II mRNA expressions were detected in KG-1 AML cell line. Relative expression of ACE I was higher than ACE II expression in this myeloid leukemic cells. Likewise, RENIN, angiotensinogen (ANGTS), and ACE I mRNA expressions were detected in HL-60 promyelocytic cell line. Relative RENIN expression was the highest, whereas ACE I expression was the lowest in HL- 60 neoplastic myeloid cells. These findings indicate that there is a biologically active local RAS in the hematopoietic system in normal and pathological states.
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