International Journal of Hematology and Oncology 2021, Vol 31, Num 4 Page(s): 205-213
Dysregulation of MS4A3 and PRDX5 Gene Expression in Multiple Myeloma Patients

Ilknur SUER1, Aynur ADAY2, Melda SARIMAN3, Mesut AYER4, Ipek Yonal HINDILERDEN2, Sema Sirma EKMEKCI5, Neslihan ABACI5, Sukru PALANDUZ1, Kıvanc CEFLE1, Sukru OZTURK1

1Istanbul University, Istanbul Medical Faculty, Department of Internal Medicine, Division of Medical Genetics, İstanbul, TURKEY
2Istanbul University, Istanbul Medical Faculty, Department of Internal Medicine, Division of Hematology, İstanbul, TURKEY
3Istinye University, Molecular Cancer Research Center, İstanbul, TURKEY
4University of Health Sciences, Haseki Training and Research Hospital, Hematology Clinic, İstanbul, TURKEY
5Istanbul University, Department of Genetics, Aziz Sancar Institute of Experimental Medicine, İstanbul, TURKEY

Keywords: Transcriptome, Multiple Myeloma, Gene Expression, MS4A3, PRDX5
Multiple myeloma (MM) is a disease in which plasma cells increase clonally. We aimed to investigate the comparison of our transcriptome data in MM, MGUS (Monoclonal gammopathy of undetermined significance) and control groups in our research. Analysis of transcriptome data revealed that CD74, FUS, MS4A3, PTPN6, PRDX5 and UNC45B genes were significantly different in the MM group compared to control group. Pathway analyzes of these genes have shown that they are associated with certain pathways such as the cellular response and immunological system. In this study, we aimed to examine the expression levels of these genes among the MM (n= 50), MGUS (n= 15) and control (n= 14) groups using the Quantitative Real-Time Polymerase Chain Reaction. According to the consequence of the study, it was determined that MS4A3 gene expression decreased significantly in the MM patient group compared to MGUS and control group, while PRDX5 gene expression was significantly increased. Also ROC (Receiver Operating Characteristic) analysis showed that MS4A3 gene has a significant diagnostic power between MM and MGUS group (area= 0.727; p= 0.008). Since multiple myeloma is more common in men than in women, it was statistically evaluated whether there is no difference in gene expression between women and men. However, it was determined that there was no statistically significant difference between the groups. As a result, the MS4A3 and PRDX5 genes, which are important in various diseases such as cancer, may shed light on new treatment options for MM disease.