International Journal of Hematology and Oncology 2019, Vol 29, Num 4 Page(s): 219-226
Role of the Rho/Rho-kinase Pathway in Endometrial Adenocarcinoma Cell Proliferation

Rukiye Nalan TIFTIK1, Ismail UN1, Derya YETKIN1, Kansu BUYUKAFSAR2

1Mersin University Faculty of Medicine, Department of Medical Pharmacology, Mersin, TURKEY
2Mersin University Faculty of Medicine, Department of Histology and Embryology, Mersin, TURKEY

Keywords: Endometrial adenocarcinoma, HEC-1A, Rho/ROCK pathway, Proliferation
Present study investigated the possible role of the Rho/ROCK pathway in the proliferation of endometrial adenocarcinoma HEC-1A cells. Cell proliferation was monitored using the xCELLigence system. HEC-1A cells were incubated with a ROCK inhibitor, Y-27632 (10−7–10−5 M) in the presence and absence of the RhoA activators, lysophosphatidic acid (LPA; 10zsup>−6 M) and calpeptin (10−6 M). The cell index was evaluated after incubation for 60, 72, 84, 96, and 108 h. Both LPA and calpeptin significantly increased cell proliferation at 60–108 h, which could be reversed by treatment with 10−5 M (but not 10−7 or 10−6 M) Y-27632. On the other hand, Y27632 (10−7–10−5 M) alone increased cell proliferation at all the time points tested. Conclusion: The Rho activators, LPA and calpeptin, induced cell proliferation in HEC-1A cells at least in part via ROCK. ROCK inhibitor, Y-27632, reversed the effects of LPA and calpeptin and increased the cell index. Further studies are required to investigate this.