International Journal of Hematology and Oncology 2018, Vol 28, Num 3 Page(s): 147-153
Effect of Resveratrol on Apoptosis and MDM2, RUNX3, RB Gene Expressions in Human Acute Myeloid Leukemia Cells by Transfection of MATRA-Mediated miR-150

Tugce B. OKCANOGLU1, Sunde Y. SUSLUER2, Cagla KAYABASI2, Besra O. YELKEN2, Guray SAYDAM3, Cigir B. AVCI2, Cumhur GUNDUZ2

1Near East University, Vocational School of Health Services, Medical Biology Department, Lefkosa, North Cyprus
2Ege University, Faculty of Medicine, Department of Medical Biology, Izmir, TURKEY
3Ege University, Faculty of Medicine, Department of Hematology, Izmir, TURKEY

Keywords: miR-150, Acute myeloid leukemia, MATRA, Resveratrol, gene expressions
Acute myeloid leukemia (AML) is a heterogeneous group of hematopoietic malignancies. Magnet assisted transfection (MATRA) is one of the most effective non-viral transfection methods. We aimed to evaluate the apoptotic effect of resveratrol (RES), MDM2, RUNX3, RB gene expression exchanges and MicroRNA- 150 (miR-150) transfected with MATRA to HL-60 cells. MATRA was used as non-viral vector carrier for miR-150 transfection. IC50 dose of RES was determineted by Fahri et al. Resveratrol and miR-150 transfected cells were performed apoptosis and MDM2, RUNX3, RB gene expression assays in Human promyelocytic leukemia (HL-60) cells. IC50 dose of RES was used as 5 μM. In HL-60 cells, it was found that miR-150, miR150-Resveratrol combination, resveratrol alone induces apoptosis by 6.48, 6.93 and 4.54 fold, respectively, compared to the control cells. Compared to control cells, MDM2, RUNX3, RB, gene expressions decreased (miR-150) 1.7, 1.3 and 1.4 fold, (miR-150-resveratrol) MDM2 expression increased 2.73 fold, RUNX3 and RB expression decreased 6.1, 1.07 fold, respectively. Combination with resveratrol, MDM2, RB decreased 2.9 and 1.4 fold, respectively. Non-viral miR-150 transfection may be effective in leukemia cells, induction of apoptotic effects and gene expression changes, following treatment with resveratrol and miR-150-resveratrol combinations.