International Journal of Hematology and Oncology
2023, Vol 33, Num 4 Page(s): 21-28
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Association Between PON1 L55M Polymorphism and PON1 Enzyme Activity in Patients with Leukemia
Nazan ERAS1, Anil TOMBAK2, Naci TIFTIK2, Serap YALIN3, Mehmet BERKOZ4, Seval KUL5, Etem AKBAS6
1Mersin University, Faculty of Medicine, Department of Medical Genetics, Mersin, TURKEY
2Mersin University, Faculty of Medicine, Department of Hematology, Mersin, TURKEY
3Mersin University, Faculty of Pharmacy, Department of Biochemistry, Mersin, TURKEY
4Yuzuncu Yil University, Faculty of Pharmacy, Department of Pharmaceutical Technology, Van, TURKEY
5Gaziantep University, Faculty of Medicine, Department of Biostatistics, Gaziantep, TURKEY
6Mersin University, Faculty of Medicine, Department of Medical Biology, Mersin, TURKEY
Keywords: Antioxidant, Leukemia, Oxidative stress, Paraoxonase (PON), Polymorphism
Paraoxonase 1 (PON1) is an important antioxidant enzyme which has a role in preventing the effects of systemic oxidative stress. The purpose of our study was to investigate the possible association between PON1 L55M polymorphism and leukemia development and to determine the relationship between PON1 genotypes and PON1 enzyme activities. Genotypes of 102 cases and 112 healthy controls were determined by PCR-RFLP. PON1 enzyme activity was determined according to Eckerson’s method. The ratio of MM genotype belonging to PON1 L55M polymorphism in control group was 6.3% and was 7.8% in patients with leukemia (p= 0.39). PON1 enzyme activity was 118.8 ± 115.1 U/mL in control group, while decreased to 75.6 ± 64.4 U/mL in patients with leukemia (p= 0.004). PON1 enzyme activities of the individuals with MM genotypes belonging to PON1 L55M polymorphism was 57.43 ± 21.61 U/ mL in control group and decreased to 39.18 ± 45.61 U/mL in leukemic patients. Our results suggest that, PON1 L55M polymorphism genotype ratios do not affect leukemia development. However, reduced PON1 enzyme activity and also the combination of PON1 L55M polymorphism with reduced PON1 enzyme activity are associated with the increased risk of leukemia. Furthermore, older age may be a risk factor for developing leukemia.
Nazan ERAS1, Anil TOMBAK2, Naci TIFTIK2, Serap YALIN3, Mehmet BERKOZ4, Seval KUL5, Etem AKBAS6
1Mersin University, Faculty of Medicine, Department of Medical Genetics, Mersin, TURKEY
2Mersin University, Faculty of Medicine, Department of Hematology, Mersin, TURKEY
3Mersin University, Faculty of Pharmacy, Department of Biochemistry, Mersin, TURKEY
4Yuzuncu Yil University, Faculty of Pharmacy, Department of Pharmaceutical Technology, Van, TURKEY
5Gaziantep University, Faculty of Medicine, Department of Biostatistics, Gaziantep, TURKEY
6Mersin University, Faculty of Medicine, Department of Medical Biology, Mersin, TURKEY
Keywords: Antioxidant, Leukemia, Oxidative stress, Paraoxonase (PON), Polymorphism
Paraoxonase 1 (PON1) is an important antioxidant enzyme which has a role in preventing the effects of systemic oxidative stress. The purpose of our study was to investigate the possible association between PON1 L55M polymorphism and leukemia development and to determine the relationship between PON1 genotypes and PON1 enzyme activities. Genotypes of 102 cases and 112 healthy controls were determined by PCR-RFLP. PON1 enzyme activity was determined according to Eckerson’s method. The ratio of MM genotype belonging to PON1 L55M polymorphism in control group was 6.3% and was 7.8% in patients with leukemia (p= 0.39). PON1 enzyme activity was 118.8 ± 115.1 U/mL in control group, while decreased to 75.6 ± 64.4 U/mL in patients with leukemia (p= 0.004). PON1 enzyme activities of the individuals with MM genotypes belonging to PON1 L55M polymorphism was 57.43 ± 21.61 U/ mL in control group and decreased to 39.18 ± 45.61 U/mL in leukemic patients. Our results suggest that, PON1 L55M polymorphism genotype ratios do not affect leukemia development. However, reduced PON1 enzyme activity and also the combination of PON1 L55M polymorphism with reduced PON1 enzyme activity are associated with the increased risk of leukemia. Furthermore, older age may be a risk factor for developing leukemia.
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