International Journal of Hematology and Oncology 2021, Vol 31, Num 1 Page(s): 149-157
Immunohistochemical Expressions of the Antimicrobial Peptides (hBD-3 and hCAP-18/LL-37) in Colon, Stomach and Lung Adenocarcinomas

Murat KILIC1, Serpil OGUZTUZUN2, Gulcin SIMSEK3, Ebru CAKIR4

1Ankara University, Vocational School of Health Services, Department of Pharmacy Services, Ankara
2Kirikkale University, Faculty of Arts and Sciences, Department of Biology, Kirikkale
3Kecioren Training and Research Hospital, Department of Pathology, Ankara
4Inonu University, Faculty of Medicine, Department of Pathology, Malatya. TURKEY

Keywords: Adenocarcinoma, hBD-3, hCAP-18/LL-37, Immunohistochemistry
This study investigated the immunohistochemical staining characteristics of human beta defensin-3 (hBD-3) and human cationic antimicrobial peptide-18/cathelicidin (hCAP-18/LL-37) in colon, stomach and lung adenocarcinomas and normal tissues (periphery to tumor tissues) from 22, 24 and 24 patients, respectively. Expressions of hBD-3 and hCAP-18/LL-37 were assessed by immunohistochemistry for colon, stomach and lung adenocarcinomas of 70 patients from Atatürk Chest Diseases and Thoracic Surgery Training and Research Hospital and Keçiören Training and Research Hospital. both located in Ankara, Turkey. The differences between the expressions of hBD-3 and hCAP-18/LL-37 in normal and carcinoma tissues were analyzed by Mann-Whitney U Test. When the normal and tumor tissues of these cases were compared according to their staining intensity of positive staining. the hBD-3 and hCAP-18/ LL-37 expressions in colon, stomach and lung adenocarcinomas cells were significantly higher than those in normal cells (p< 0.05). Immunostaining of HBD-3 and hCAP-18/LL-37 was found to be a marker of malignancy in colon. stomach and lung adenocarcinomas. The expressions of hBD-3 and hCAP-18/LL-37 were, for the first time, shown to be significantly altered in colon, stomach and lung adenocarcinomas as compared to controls. In conclusion, the present findings suggest that beside the antimicrobial activity of Antimicrobial Peptides (AMPs), hBD-3 and hCAP-18/LL-37 can also play a role in the pathogenesis of colon. stomach and lung adenocarcinomas.