International Journal of Hematology and Oncology 2021, Vol 31, Num 4 Page(s): 125-133


Akdeniz University, Art and Science Faculty, Biology Department, ANTALYA

Keywords: nsclc, epirubicin-hci, gsh, gst, gsh-px
Epirubicin-HCl as many cytotoxic drugs generates both superoxide and hydrogen peroxide. We assessed the relative significance of catalase, SOD, GSH, GST, GSH-Px and GST-Pi in protecting these cell against epirubicin-HCI cytotoxicity. NSCLC cells were grown in the absence and presence of increasing concentrations of epirubicin-HCI. The cytotoxicity of epirubicin-HCI was measured using the MTT cytotoxicity test on viability of NSCLC cells. Epirubicin-HCI produced a concentration and time dependent cytotoxicity in NSCLC cells. The IC50 value of epirubicin-HCI 1.2 µg/ml, caused 51.27% cytotoxicity in 24 hours and exhibited increasing cytotoxicity (69.15%) in 48 hours incubation. The cytotoxicity of epirubicin-HCI (IC50 value of 1.2 µg/ml in 24 hours, 0.8 µg/ml in 48 hours and 0.62 mg/ml in 72 hours) appeared to involve a free radical species and hidrojen peroxide production type of mechanism since enzymes activity of GST, GSH-Px , amount of GSH and expression of GST-Pi were increased in 24 hour incubation. Addition of SOD and catalase prevented cytotoxicity of epirubicin-HCI. It is proposed that, production of reactive oxygen species and hydrogen peroxide by the treatment of epirubicin-HCI can cause cytotoxicity of NSCLC cells. SOD, catalase, GSH-Px, GST, GST-Pi, and GSH must be considered as part of the intracellular antioxidant defense mechanism of NSCLC cells against to single electron reducing quinone-containing anticancer antibiotics such as epirubicin-HCI.