International Journal of Hematology and Oncology
2023, Vol 33, Num 4 Page(s): 275-281
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Histopathological Alterations in the Kidney Tissue Following Topical Ankaferd Hemostat Application in a Rat Renal Injury Model
Derya KARAKOC1, Eylem AKAR2, Salih AKSU3, Aysegul UNER4, Arif OZDEMIR1, Erhan HAMALOGLU1, Ahmet OZENC1, Ahmet DOGRUL1, Bibihanim MEMEDOVA1, Ibrahim C. HAZNEDAROGLU3
1Hacettepe University Faculty of Medicine, Department of General Surgery, Ankara, TURKEY
2Hacettepe University Faculty of Medicine, Department of Pathology, Ankara, TURKEY
3Hacettepe University Faculty of Medicine, Department of Hematology, Ankara, TURKEY
4Hacettepe University Faculty of Medicine, Department of Pathology, Ankara, TURKEY
Keywords: Ankaferd Blood Stopper, Renal Injury, Erythrocyte Aggregation, Siderophages Accumulation
Ankaferd Blood Stopper (ABS) is a novel topical hemostatic agent. ABS has been approved in Turkey for clinical hemorrhages, when the conventional control of bleeding by ligature and/or conventional hemostatic measures is ineffective. ABS has many cellular effects and could modulate numerous hemostatic proteins at the tissue and blood. ABS-induced formation of the protein network with vital erythroid aggregation covers the entire physiological hemostatic process. The aim of this study was to assess histopathological alterations due to topical ABS administration at the renal tissue level. Thirty-six Wistar rats weighing 70 to 80 gm were included into the study. The rats were divided into two groups as “the ABS-applied group” (ABS−G) and “the control group” (C−G). The animals in both groups were then again divided into the three subgroups of “postoperative (Postop.) 60th minutes”, “Postop. 48th hours”, and “Postop. 15th day”. Therefore, there were six rats in each of the subgroups at the end of the analyses. The standard renal injury sites in the rats of ABS−G were applied 2 ml. of topical ABS, whereas 2 ml. of topical saline was applied to the renal injury sites of the rats in the C−G group. We detected significant erythrocyte aggregation and the accumulation of siderophages in the kidney tissue just after 60 minutes of ABS application persisting over 15 days. Our results indicated red blood cell accumulation and siderophages following the use of ABS are compatible with the suggested ‘mechanism-of-action’ of ABS that ABS-induced formation of the protein network with vital erythroid aggregation covers the entire physiological hemostatic process. Further experimental search is needed to find out the molecules inside the ABS protein library leading to the ABS-induced aggregation at the renal tissue level.
Derya KARAKOC1, Eylem AKAR2, Salih AKSU3, Aysegul UNER4, Arif OZDEMIR1, Erhan HAMALOGLU1, Ahmet OZENC1, Ahmet DOGRUL1, Bibihanim MEMEDOVA1, Ibrahim C. HAZNEDAROGLU3
1Hacettepe University Faculty of Medicine, Department of General Surgery, Ankara, TURKEY
2Hacettepe University Faculty of Medicine, Department of Pathology, Ankara, TURKEY
3Hacettepe University Faculty of Medicine, Department of Hematology, Ankara, TURKEY
4Hacettepe University Faculty of Medicine, Department of Pathology, Ankara, TURKEY
Keywords: Ankaferd Blood Stopper, Renal Injury, Erythrocyte Aggregation, Siderophages Accumulation
Ankaferd Blood Stopper (ABS) is a novel topical hemostatic agent. ABS has been approved in Turkey for clinical hemorrhages, when the conventional control of bleeding by ligature and/or conventional hemostatic measures is ineffective. ABS has many cellular effects and could modulate numerous hemostatic proteins at the tissue and blood. ABS-induced formation of the protein network with vital erythroid aggregation covers the entire physiological hemostatic process. The aim of this study was to assess histopathological alterations due to topical ABS administration at the renal tissue level. Thirty-six Wistar rats weighing 70 to 80 gm were included into the study. The rats were divided into two groups as “the ABS-applied group” (ABS−G) and “the control group” (C−G). The animals in both groups were then again divided into the three subgroups of “postoperative (Postop.) 60th minutes”, “Postop. 48th hours”, and “Postop. 15th day”. Therefore, there were six rats in each of the subgroups at the end of the analyses. The standard renal injury sites in the rats of ABS−G were applied 2 ml. of topical ABS, whereas 2 ml. of topical saline was applied to the renal injury sites of the rats in the C−G group. We detected significant erythrocyte aggregation and the accumulation of siderophages in the kidney tissue just after 60 minutes of ABS application persisting over 15 days. Our results indicated red blood cell accumulation and siderophages following the use of ABS are compatible with the suggested ‘mechanism-of-action’ of ABS that ABS-induced formation of the protein network with vital erythroid aggregation covers the entire physiological hemostatic process. Further experimental search is needed to find out the molecules inside the ABS protein library leading to the ABS-induced aggregation at the renal tissue level.
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