International Journal of Hematology and Oncology 2019, Vol 29, Num 1 Page(s): 170-175
Effects Of Thalidomide And rhGM-CSF on Carbohydrate Metabolism In HL-60 Acute Promyelocytic Leukemia Cell Line

Hakan BOYUNAGA1, Günnur DIKMEN1, A. Ugur URAL1, Abdullah MELEKOGLU1

1Kirikkale University, Faculty of Medicine, Department of Biochemistry, Kirikkale, TURKEY
2Hacettepe University, Faculty of Medicine, Department of Biochemistry, Ankara, TURKEY
3Gulhane Faculty of Medicine, Department of Hematology, Ankara, TURKEY
4Kirikkale University, Faculty of Science and Arts, Department of Biology Kirikkale, TURKEY

Keywords: Thalidomide, Metabolism, rhGM-CSF, HL-60 cell line
The purpose of this study is to investigate the in vitro effect of thalidomide and rhGM-CSF on energy metabolism rate in HL-60 cell lines. HL-60 cells were incubated with radiolabelled glucose for 4 hours. Following incubation, radiolabelled CO2 was isolated and collected in specially designed scintillation vials. The other end-products of carbohydrate catabolism collected via anion-exchange chromatography were analyzed using liquid scintillation. Protein and glycogen levels for each group were also determined. The study results indicated that the use of drugs for 2-days had no significant effect when compared with aerobic and anaerobic controls. However, groups treated with the drugs for 5 days showed significant differences. It was obvious that rhGM-CSF caused HL-60 leukemia cells to use aerobic glycolytic pathway for production of energy. When thalidomide and rhGM-CSF combination therapy results were compared with untreated control cells, it was observed that glycogen consumption was decreased by 50.37%, and CO2 production was increased by 94.03%. When compared with those of anaerobic controls, glycogen consumption and CO2 production rates were found to be decreased by 54.01% and increased by 96.59%, respectively. As a consequence, we found that those cell lines treated with combined use of Thalidomide and rhGM-CSF caused HL-60 leukemia cells to predominantly prefer aerobic glycolytic pathways for energy production.