International Journal of Hematology and Oncology 2019, Vol 29, Num 2 Page(s): 018-022
Determination of ApaI and TaqI Polymorphisms of VDR Gene in a Group of Turkish Patients with Colorectal Cancer

Fuat DİLMEÇ1, Abdullah ÖZGÖNÜL2, Feridun AKKAFA1, Ali UZUNKOY2, André B.P. van KUILENBURG3

1Harran University, Faculty of Medicine, Department of Medical Biology, ŞANLIURFA
2Harran University, Faculty of Medicine, Department of General Surgery, ŞANLIURFA
3University of Amsterdam, Emma Children’s Hospital and Department of Clinical Chemistry, Academic Medical Center, AMSTERDAM

Keywords: VDR gene, Colorectal cancer, Polymorphism, PCR, RFLP
Colorectal cancer (CRC) is the leading cause of cancer death among human around the world. The vitamin D receptor gene (VDR) is a member of the nuclear receptor super family, which is expressed in the tissue of gastrointestinal tract, known to modulate the rate of cell proliferation. We aimed to investigate the genotype and allele frequencies and association of the VDR gene: c.1025-49G>T (ApaIG>T) and c.1056T>C (TaqIT>C) polymorphisms with CRC in Turkish patients. Fifty-six patients with CRC and 169 healthy individuals were enrolled to study, and their DNA was isolated. A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used to detect the frequency of c.1025-49G>T and c.1056T>C polymorphisms. The prevalence of the c.1025-49G>T and c.1056T>C alleles and the genotype frequencies in patients with CRC was similar to that in the normal population. The investigated polymorphisms in the VDR gene do not represent a significant risk factor for CRC in our population.