International Journal of Hematology and Oncology
2023, Vol 33, Num 4 Page(s): 212-216
THE COMPARISON OF CSA WHOLE BLOOD LEVELS USING TDX-M, EMIT AND AXSYM IMMUNOASSAYS
SELVİ AYGÜN KAYA1, TÜLAY KILIÇASLAN AYNA1, HAYRİYE ŞENTÜRK1, HANDAN AĞIRBAŞLI1, UĞUR AKAR1, MEHMET GİRTEKİN1, NEVİN YALMAN1, SEMA ANAK1, HÜLYA BİLGEN1, MAHMUT N. ÇARİN1, GÜNDÜZ GEDİKOĞLU1
İstanbul Üniversitesi, İstanbul Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, İSTANBUL
Keywords: axsym, csa levels, emit, tdx-m
Our study aims to determine cyclosporine A (CsA), blood levels by using three different methods in twelve patients who had Bone Marrow Transplantation (BMT) for underlying diseases of [Acute Lymphoblastic Leukemia (ALL), Chronic Myeloblastic Leukemia (CML), Acute Myeloblastic Leukemia (AML), Myelodyplastic Syndrome (MDS) and Fanconi Aplastic Anemia (FAA)]. The CsA levels are tested by using TDx-m FPIA (Fleorescence polarization immunoassay) Abbott, EMIT (Enzyme-multiplied immunoassay technique) Behring and AxSYM FPIA Abbott methods. In the working samples, from which the CsA blood levels were measured, the levels were at the maximum using TDx-m method, at the medium using EMIT values and at the minimum using AxSYM method. Our measurements to test the precision of the three methods the differences were not meaningful statistically. Because of the CsA metabolites and cross reactions in TDx-m and AxSYM methods, the values were too high, so we traced that EMIT method is more stable, reliable, more useful and stabilized for guiding medical treatment, therefore should be preferred.
SELVİ AYGÜN KAYA1, TÜLAY KILIÇASLAN AYNA1, HAYRİYE ŞENTÜRK1, HANDAN AĞIRBAŞLI1, UĞUR AKAR1, MEHMET GİRTEKİN1, NEVİN YALMAN1, SEMA ANAK1, HÜLYA BİLGEN1, MAHMUT N. ÇARİN1, GÜNDÜZ GEDİKOĞLU1
İstanbul Üniversitesi, İstanbul Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, İSTANBUL
Keywords: axsym, csa levels, emit, tdx-m
Our study aims to determine cyclosporine A (CsA), blood levels by using three different methods in twelve patients who had Bone Marrow Transplantation (BMT) for underlying diseases of [Acute Lymphoblastic Leukemia (ALL), Chronic Myeloblastic Leukemia (CML), Acute Myeloblastic Leukemia (AML), Myelodyplastic Syndrome (MDS) and Fanconi Aplastic Anemia (FAA)]. The CsA levels are tested by using TDx-m FPIA (Fleorescence polarization immunoassay) Abbott, EMIT (Enzyme-multiplied immunoassay technique) Behring and AxSYM FPIA Abbott methods. In the working samples, from which the CsA blood levels were measured, the levels were at the maximum using TDx-m method, at the medium using EMIT values and at the minimum using AxSYM method. Our measurements to test the precision of the three methods the differences were not meaningful statistically. Because of the CsA metabolites and cross reactions in TDx-m and AxSYM methods, the values were too high, so we traced that EMIT method is more stable, reliable, more useful and stabilized for guiding medical treatment, therefore should be preferred.