International Journal of Hematology and Oncology
2023, Vol 33, Num 4 Page(s): 207-211
MAJOR ANTIOXIDANT ENZYME STATUS IN MCF-10A CELL LINES TREATED WITH 17-BETA-ESTRADIOL AND 7,12-DIMETHYLBENZ(A)ANTHRACENE: A PRELIMINARY STUDY
ÖMER AKYOL1, AHMET GÜREL1, HALUK AKIN1
New York University Medical Center, Department of Environmental Medicine, Laboratory on Carcinogenesis, NewYork
Keywords: mcf-10a cell line, estradiol, dmba, catalase, superoxide dismutase, glutathione peroxidase
There is a great evidence within the past few years that oxidative stress has an important role in the process of carcinogenesis. Exposure of MCF-10A cells (human breast epithelial cell line) to the mammary carcinogen 7,12-dimethylbenz (a)anthrecene (DMBA) and possible tumor promoter 17- beta -estradiol (E2) may affect the neoplastic transformation of cells leading to production of reactive oxygen species (ROS). The effects of E2 and DMBA on free radical scavenging enzyme activities have been investigated in MCF-10A cell lines in this study. MCF-10 cells were treated with 0 µM, 10 µM, 25 µM DMBA, and 0 M, 10-9 M, 10-8 M E2 for 4, 8, and 12 weeks in cell culture media. After these exposure periods, cells were homogenized in hypotonic solution with Triton X-100. Superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) activities were determined in these cell extracts and compared with each other. There is an increase in GSH-Px activity in cells treated with DMBA (10 µM) and E2 (10-9 M) for 12 weeks compared with control cells (treated with only acetone 0.1% and ethanol 0.01%, respectively, for 12 weeks). SOD and CAT activities were changed significantly in a irregular manner depending on concentrations of DMBA and E2, and exposure time.
ÖMER AKYOL1, AHMET GÜREL1, HALUK AKIN1
New York University Medical Center, Department of Environmental Medicine, Laboratory on Carcinogenesis, NewYork
Keywords: mcf-10a cell line, estradiol, dmba, catalase, superoxide dismutase, glutathione peroxidase
There is a great evidence within the past few years that oxidative stress has an important role in the process of carcinogenesis. Exposure of MCF-10A cells (human breast epithelial cell line) to the mammary carcinogen 7,12-dimethylbenz (a)anthrecene (DMBA) and possible tumor promoter 17- beta -estradiol (E2) may affect the neoplastic transformation of cells leading to production of reactive oxygen species (ROS). The effects of E2 and DMBA on free radical scavenging enzyme activities have been investigated in MCF-10A cell lines in this study. MCF-10 cells were treated with 0 µM, 10 µM, 25 µM DMBA, and 0 M, 10-9 M, 10-8 M E2 for 4, 8, and 12 weeks in cell culture media. After these exposure periods, cells were homogenized in hypotonic solution with Triton X-100. Superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) activities were determined in these cell extracts and compared with each other. There is an increase in GSH-Px activity in cells treated with DMBA (10 µM) and E2 (10-9 M) for 12 weeks compared with control cells (treated with only acetone 0.1% and ethanol 0.01%, respectively, for 12 weeks). SOD and CAT activities were changed significantly in a irregular manner depending on concentrations of DMBA and E2, and exposure time.