International Journal of Hematology and Oncology
2023, Vol 33, Num 4 Page(s): 144-150
EVALUATION OF PERIPHERAL BLOOD PLATELET ACTIVATION AND LEUKOCYTE-PLATELET AGGREGATES IN RELATION TO DISEASE ACTIVITY IN INFLAMMATORY BOWEL DISEASES
İLHAN ÖZTOP1, FATİH DEMİRKAN1, G. HAYRİ ÖZSAN1, HALİL ATEŞ1, MİNE ÇABUK1, BÜLENT ÜNDAR1
Dokuz Eylül University, Medical Faculty, Division of Hematology, İZMİR
Keywords: inflammatory bowel diseases, p-selectin, circulating leukocyte-platelet aggregates, flow-cytometry
Platelets may exhibit proinflammatory as well as prothrombotic properties. We studied platelet activation and circulating leukocyte-platelet aggregates(LPAs) by a flow cytometric assay in inflammatory bowel diseases (IBD). Three Crohn's disease(CD) and 12 ulcerative colitis (UC) (7 with active disease) patients and 15 healthy controls formed the study group. Activated platelets and circulating LPAs were identified by CD42b/CD62P and CD45/CD42b positivity, respectively. P-selectin (CD62P) expression and circulating LPAs were higher in patients with IBD compared to control group (as mean value ± standard error of mean, 4.53%±0.80 vs 1.61%±0.43, P<0.05 and 5.72%±0.97 vs 1.87%±0.14, p<0.05, respectively). When active and inactive patients were compared for both parameters, there was no statistical significance. However, when inactive patients were compared with the control group, we detected a significance for circulating LPAs (p<0.05) but not for P-selectin expression. Flow-cytometric assays for circulating LPAs might be an early activation marker for IBD patients who exacerbates frequently.
İLHAN ÖZTOP1, FATİH DEMİRKAN1, G. HAYRİ ÖZSAN1, HALİL ATEŞ1, MİNE ÇABUK1, BÜLENT ÜNDAR1
Dokuz Eylül University, Medical Faculty, Division of Hematology, İZMİR
Keywords: inflammatory bowel diseases, p-selectin, circulating leukocyte-platelet aggregates, flow-cytometry
Platelets may exhibit proinflammatory as well as prothrombotic properties. We studied platelet activation and circulating leukocyte-platelet aggregates(LPAs) by a flow cytometric assay in inflammatory bowel diseases (IBD). Three Crohn's disease(CD) and 12 ulcerative colitis (UC) (7 with active disease) patients and 15 healthy controls formed the study group. Activated platelets and circulating LPAs were identified by CD42b/CD62P and CD45/CD42b positivity, respectively. P-selectin (CD62P) expression and circulating LPAs were higher in patients with IBD compared to control group (as mean value ± standard error of mean, 4.53%±0.80 vs 1.61%±0.43, P<0.05 and 5.72%±0.97 vs 1.87%±0.14, p<0.05, respectively). When active and inactive patients were compared for both parameters, there was no statistical significance. However, when inactive patients were compared with the control group, we detected a significance for circulating LPAs (p<0.05) but not for P-selectin expression. Flow-cytometric assays for circulating LPAs might be an early activation marker for IBD patients who exacerbates frequently.