International Journal of Hematology and Oncology 2019, Vol 29, Num 3 Page(s): 055-060
EVALUATION OF PLATELET AGGREGATION BY IMPEDANCE AND OPTIC METHODS IN HEALTHY CHILDREN

NİMET KABAKUŞ1, BAYRAM YILMAZ1, AHMET AYAR1

Departments of Pediatrics, Fırat University, Faculty of Medicine, ELAZIĞ

Keywords: platelet aggregation, optic method, impedance method, childhood
Platelet aggregation (PA) may be affected by congenital and acquired factors (food, age, sex, metabolic state and biochemical constituents of blood). The present study was undertaken to investigate PA in 31 healthy children by optic [plasma group: 6 boys, 10 girls; mean age (mean ± SD) 17.9 ± 3.4 months, range: 1-36] and impedance [whole blood group: 6 boys, 9 girls; mean age (mean ± SD): 16.7 ± 4.2 months, range: 2-44] methods. Template bleeding times were measured by Ivy method in all children. In the platelet rich plasma, epinephrine-induced PA was suppressed (<25%) in most of the children (10/16, 62.5%). In this group, adenosine diphosphate (ADP)-, collagen- and ristocetin-induced PA values were between 25% and 50% (56.3%-9/16, 43.8%-7/16 and 68.8%-11/16), respectively. In the whole blood, ADP- (80%-12/15), collagen- (80%-12/15) and ristocetin-induced PA (86.6%-13/15) values were between 51% and 100% in most of the children. There was no response to ADP-induced PA in only one child (6.7%) in this group. PA % values were lower than those reported in children and adults in the literature. Collagen-induced PA-bleeding times and PA-Mean corpuscular volume were positively correlated in the whole blood group. Epinephrine-induced PA-bleeding times were also positively correlated in the PRP group. All agonists were synergistic to increase PA. These findings indicate that PA results may vary depending on age, dietary habitat and methods employed.