International Journal of Hematology and Oncology
2023, Vol 33, Num 4 Page(s): 031-035
D5S679-6 SEEMS TO BE AN INFORMATIVE MICROSATELLITE MARKER FOR SMALL CELL LUNG CANCER
SACİDE PEHLİVAN1, MUSTAFA PEHLİVAN1, MERAL KOYUNCUOĞLU1, ALİ ÖZCAN1, ATİLLA AKKOÇLU1
Ege Üniversitesi Fen Fakültesi Moleküler Biyoloji AD, İZMİR
Keywords: small-cell lung cancer, microsatellite instability, and 5ql3
Microsatellite instability has been rarely reported in small-cell lung cancer (SCLC). Meanwhile, loss of heterozygosity (LOH) was found in certain amounts on some dinucleotide repeats situated on chromosomal locations 1p, 3p. 5q, 8p,9p, 10p, 11p, 13q, 22q and 17. We studied the presence of specific dinucleotide repeat (D5S679) on 5ql 3 for instability, the presence of LOH and diverse alleles specific to our population as well as their frequency. The allele number six of D5S679 was found in 25 of 27 (92.6%) cases analyzed in our study. However, the same allele was encountered only in two of 39 (2.6%) individuals in normal control group (p<0.0001). When tumor tissue was compared to non-tumor lung tissue in only six cases among 27 cases with SCLC, LOH was found in four and microsatellite instability in two. A study of D5S679 in SCLC was not reported previously. The high frequency of the allele 6 of D5S679 in SCLC shows that it could be a significant marker for this disease and it contains a specific allel within SCLC in Turkish population. In conclusion, this microsatellite is an appropriate parameter for MI and LOH studies.
SACİDE PEHLİVAN1, MUSTAFA PEHLİVAN1, MERAL KOYUNCUOĞLU1, ALİ ÖZCAN1, ATİLLA AKKOÇLU1
Ege Üniversitesi Fen Fakültesi Moleküler Biyoloji AD, İZMİR
Keywords: small-cell lung cancer, microsatellite instability, and 5ql3
Microsatellite instability has been rarely reported in small-cell lung cancer (SCLC). Meanwhile, loss of heterozygosity (LOH) was found in certain amounts on some dinucleotide repeats situated on chromosomal locations 1p, 3p. 5q, 8p,9p, 10p, 11p, 13q, 22q and 17. We studied the presence of specific dinucleotide repeat (D5S679) on 5ql 3 for instability, the presence of LOH and diverse alleles specific to our population as well as their frequency. The allele number six of D5S679 was found in 25 of 27 (92.6%) cases analyzed in our study. However, the same allele was encountered only in two of 39 (2.6%) individuals in normal control group (p<0.0001). When tumor tissue was compared to non-tumor lung tissue in only six cases among 27 cases with SCLC, LOH was found in four and microsatellite instability in two. A study of D5S679 in SCLC was not reported previously. The high frequency of the allele 6 of D5S679 in SCLC shows that it could be a significant marker for this disease and it contains a specific allel within SCLC in Turkish population. In conclusion, this microsatellite is an appropriate parameter for MI and LOH studies.