International Journal of Hematology and Oncology
2023, Vol 33, Num 4 Page(s): 171-177
COMPARISON OF TWO CRYOPRESERVATION METHOD ACCORDING TO STEM CELL VIABILITY AND COLONY FORMING CAPACITY
S. SAMİ KARTI1, CAFER ADIGÜZEL1, MUSTAFA YILMAZ1, MUSTAFA ÇETİNER1, SİRET RATİP1, EMEL EKŞİOĞLU1, SEMA AKTAŞ1, NEBİYE SOFU1, YEŞİM ELBİR1, ERCÜMENT OVALI1, MAHMUT BAYIK1
Karadeniz Teknik Üniversitesi, Tıp Fakültesi, Hematoloji BD, TRABZON
Keywords: stem cell, cryopreservation, programmed freezing, mechanical freezing, viability, cfu-mix
Peripheral blood stem cells (PBSCs) are usually cryopreserved with rate-controlled programmed freezing (RCPF). However, this method is expensive and time consuming. A simplified method named mechanical freezing (MF) for cryopreservation of PBSCs at -800C without rate-controlled freezing has been developed for cryopreservation. In this study cell viability, colony forming potential and CD34(+) cell loss after thawing of cryopreserved PBSC with RCPF and MF methods were compared. Six subject's PBSCs were cryopreserved with RCPF while 19 subject's PBSCs were cryopreserved with MF. There was no significant difference between the cell viability and 14th day colony count, just after and 1 hour after thawing in RCPF. The cell viability and colony forming potential of PBSCs, cryopreserved with MF decreased significantly one hour after thawing. When the two methods are compared, the cell viability and colony forming potential was better with RCPF just after and 1 hour after thawing. In conclusion, we observed that in RCPF the viability and hematopoietic activity of PBSC is well preserved and the stem cell viability does not decrease in room temperature for one hour. In the light of these results it is suggested to continue cryopreservation with RCPF.
S. SAMİ KARTI1, CAFER ADIGÜZEL1, MUSTAFA YILMAZ1, MUSTAFA ÇETİNER1, SİRET RATİP1, EMEL EKŞİOĞLU1, SEMA AKTAŞ1, NEBİYE SOFU1, YEŞİM ELBİR1, ERCÜMENT OVALI1, MAHMUT BAYIK1
Karadeniz Teknik Üniversitesi, Tıp Fakültesi, Hematoloji BD, TRABZON
Keywords: stem cell, cryopreservation, programmed freezing, mechanical freezing, viability, cfu-mix
Peripheral blood stem cells (PBSCs) are usually cryopreserved with rate-controlled programmed freezing (RCPF). However, this method is expensive and time consuming. A simplified method named mechanical freezing (MF) for cryopreservation of PBSCs at -800C without rate-controlled freezing has been developed for cryopreservation. In this study cell viability, colony forming potential and CD34(+) cell loss after thawing of cryopreserved PBSC with RCPF and MF methods were compared. Six subject's PBSCs were cryopreserved with RCPF while 19 subject's PBSCs were cryopreserved with MF. There was no significant difference between the cell viability and 14th day colony count, just after and 1 hour after thawing in RCPF. The cell viability and colony forming potential of PBSCs, cryopreserved with MF decreased significantly one hour after thawing. When the two methods are compared, the cell viability and colony forming potential was better with RCPF just after and 1 hour after thawing. In conclusion, we observed that in RCPF the viability and hematopoietic activity of PBSC is well preserved and the stem cell viability does not decrease in room temperature for one hour. In the light of these results it is suggested to continue cryopreservation with RCPF.